A Simple Test of Seminal Fluid Chemistry and Its Potential Impact on Cryopreservation

Cryopreservation is currently the only effective tool for long-term storage of semen in most species. However, it well-recognized that, even species that freeze well, some individuals resist cryopreservation. Work from this laboratory has demonstrated a relationship between maternal lipid content and chemical constitution embryos they produce. The objective present study was to determine if similar might exist paternal body chemistry animal’s sample such difference could be determined with simple weight test. Semen samples were obtained cattle known differences composition. first underwent analysis then prepared as either cell-free (CF) or neat specimens (NS). Known volumes each weighed, remainder analyzed lipids, total proteins, carbohydrates using series spectrophotometric assays blood techniques. As expected, seen CF vs NS preparations individual (p < 0.001). Differences also found triglycerides 0.001), glucose protein fructose 0.009) differing Statistical suggested non-linear correlation observed weights 0.047) well triglyceride levels 0.003). Together, these data suggest possible develop an algorithm allow adjustment cryoprotectants based on procedure, allowing modification basis potentially improving outcomes valuable animals classified “poor freezers”.